William M. Geisler, Robert J. Suchland, William L. H. Whittington, Walter Elizabeth. Stamm, Decimal Culture out of Chlamydia trachomatis: Dating out of Introduction-Developing Tools Made in Community to help you Systematic Symptoms and you may Acute Tenderness from inside the Urogenital Problem, The fresh Journal regarding Contagious Illness, Frequency 184, Situation 10, , Profiles 1350–1354,
Abstract
The partnership away from Chlamydia trachomatis addition-developing units in quantitative people to medical symptoms and pain during the urogenital state was assessed inside 1179 patients probably a sexually carried illness medical center. 450 ifu), number and you will character from cervical discharge, ?30 polymorphonuclear muscle (PMNL) for each large-electricity profession (hpf) toward Gram stain (2050 versus. 320 ifu), and diagnoses regarding mucopurulent cervicitis (MPC; 2550 compared to. three hundred ifu) and pelvic inflammatory problem (PID; 3000 compared to. 578 ifu). Inside guys, higher inclusion-forming equipment counts was indeed associated with the urethral launch (85 versus. 49 ifu), count and you can character out of release, and you may ?ten PMNL/hpf (95 vs. fifty ifu). These contacts carried on towards multivariate analysis. For this reason, chlamydial replication is actually regarding the MPC and you will PID in women, urethritis during the boys, and inflammation in both. As the bacterial infections with a high addition counts is the most transmissible, character and you may treatments for customers with this chlamydia-associated syndromes plays a role in control applications
Understanding the dating out-of Chlamydia trachomatis urogenital state so you’re able to systematic symptoms and you may severe pain is important in finest knowing the epidemiology and pathogenesis of them bacterial infections. Logical manifestations because of C. trachomatis probably depict the fresh joint results of head chlamydial duplication and you can host inflammatory solutions [ 1]. The relationship ranging from energetic chlamydial replication and you may clinical signs will be reviewed using the level of introduction-creating gadgets manufactured in cellphone muscle people due to the fact a quantitative measure of the number of viable contagious basic regulators when you look at the genital secretions. What amount of addition-developing systems for the culture could be thought to be an effective surrogate to possess infectivity or transmissibility [ 2]. By the use of a large databases one allowed for adjustment of prospective confounding things, i used a cross-sectional study to evaluate the relationship between chlamydial addition-building units in the quantitative societies with health-related symptoms and you may amount of severe tenderness in urogenital chlamydial disease
Actions
Patient population and you can studies collectionThe study inhabitants contains 671 people and you will 933 heterosexual people who’d a society-documented urogenital chlamydial infection at the Societal Fitness, Seattle and you may Queen Condition Intimately Transmitted Problems Clinic away from Get 1995 courtesy December 1999. Analysis study was in fact regarding clinic’s computerized databases, which has intercourse-particular standard scientific list forms. Persons having coexisting gonococcal issues otherwise problems due to nonfusing C. trachomatis phenotypes was in fact omitted as a means regarding approaching possible confounders you to definitely ydial introduction-developing device counts to help you clinical symptoms [ 3, 4]. Customers with repeat infections with similar serovar in this a month or combined serovar infection have been omitted, since their addition carry out violate freedom on multivariate studies
Source and characterization ofC. trachomatis isolatesThe methods used for specimen collection, isolation of C. trachomatis identification and quantification of inclusions, and serotyping have been described in detail elsewhere [ 2, 5]. In brief, specimens were collected, were stored in chlamydia transport medium at 4°C, and were transported within 24 h to the laboratory. Each specimen was inoculated onto McCoy cells and overlaid with growth medium. Chlamydial inclusions were detected with a genus-specific antichlamydial lipopolysaccharide monoclonal antibody conjugated with fluorescein datingranking.net/cs/beetalk-recenze/. The average inclusion count per 100-?L well was determined by counting 3 fields and multiplying the average count by a predetermined conversion factor based on magnification with a Zeiss epifluorescence microscope. Inclusions were counted individually in cultures with <100 inclusions. All technicians who determined inclusion-forming unit counts used the same standardized method [ 5]. For serotyping, chlamydial strains were cultured in 96-well microtiter plates and were serotyped by using subspecies- and serovar-specific monoclonal antibodies. Serovars were additionally grouped as B complex (serovars B, Ba, D, Da, D ? , E, L1, L2, and L2a), intermediate group FG (serovars F and G), and C complex (serovars A, C, H, I, Ia, I ? , J, K, and L3) [ 6–8]. The nonfusing chlamydial phenotype was detected visually by observing inclusion morphology during serotyping [ 5]
